Liquid Chromatography - Part of Post Diploma Certificate in Advanced Analytical Methods (SDAS)

Training Provider: Ngee Ann Polytechnic
Course Reference: TGS-2024041917
S$1,318

About This Course

Module Objectives

MO1 Describe the general principles of some techniques used in analytical, chemistry/biochemistry laboratories (UV-visible spectrophotometry and chromatography –liquid, analytical, mass selective detectors), their uses, advantages, disadvantages and limitations.
MO2 Describe briefly the workings of the instrumentation involved.
MO3 Carry out simple chemical analysis using HPLC chromatographic systems.
MO4 Interpret chromatograms and derive qualitative and quantitative information from them.
MO5 Perform simple troubleshooting and critically analyse experimental data and protocols with regard to analytical parameters such as precision, accuracy, reproducibility, sensitivity, noise, limits of detection and limits of quantitation, calibration graphs, controls and standards
MO6 Understand and apply the fundamental principles used in chemical analysis, including significant figures, mole, units, unit conversion factors, preparation of solutions, serial dilutions, dilution factor, random errors and systematic errors are introduced.
MO7 Understand and perform careful preparation of calibration standards and sample preparation.
MO8 Understand the fundamentals of analytical method validation, which includes the criteria for acceptance.

What You'll Learn

This module will cover the detailed operations of HPLC, UHPLC, and its associated detectors (DAD, UV, RI, etc), from method development (sample preparation, column selection, solvent selection, gradients, baseline correction), data analysis (internal and external standards, normalization) to method validation and troubleshooting. Participants will have industrial case studies to discuss and troubleshoot, samples to analyze and report to prepare as a group project.

Entry Requirements

-A local polytechnic diploma/degree in science or engineering with a chemistry component (e.g. Life Sciences, Chemical Sciences, Chemical Engineering).
-Applications with other qualifications and significant work experience will also be considered.

Course Details

Duration 36 hours
Language English
Training Commitment Part Time
Total Enrolled 3 students
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